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.com
Volume 7, Issue 4 (Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
Bio America 2017
October 19-20, 2017
October 19-20, 2017 | New York, USA
18
th
Biotechnology Congress
Effect of carbon to nitrogen ratio in the growth of
Schizochytrium limacinum
towards DHA production
Juan Martin Delgado, Rubén Darío Munera, Mariana Peñuela Vásquez
and
Catalina Andrea Lugo De Ossa
Universidad de Antioquia, Colombia
D
ocosahexaenoic Acid (DHA) is linked to brain and sight development in children. Many children in Colombia do not have
access to diets containing these essential nutrients. To improve this condition, it is necessary to explore different sources for
DHA. In recent years, Schizochytrium limacinum SR21 has emerged as an alternative to the production of DHA; nevertheless, this
way of productions is still underdeveloped. To cope up with this necessity, we are studying the conditions under which SR21 yields
increase by varying carbon to nitrogen ratio, as well as, lowering production costs for this technology. Varying the carbon to nitrogen
ratio allows finding the best conditions under which biomass and lipid induction happen, this is important given that S. limacinum
is not endemic to Colombia; therefore, it is necessary to establishing the optimum conditions for the region. Initially, we evaluated
a constant carbon (glucose) concentration of 50g/L varying nitrogen (yeast extract) concentrations in C:N rations of 5:1, 15:1, 25:1,
and 35:1 respectively in a 6 day fermentation at 23°C and 160 rpm. After analyzing the data, we found that the 5:1 ratio yielded 38g/L
DCW although the growth was slow and we did not reach the stationary phase, 25:1 ratio showed the overall best conditions with
26g/L DCW in 120 hours. The next step in the study will be to optimize the conditions for lipid induction and profiling the oil to
characterize the DHA produced in the fermentation process. These results are very promising for further up scaling and production
of a nutraceutical food for children enriched with DHA. Microalgae are a great candidate to shift the production of DHA from fish
capture to renewable sources; moreover, new biotechnological sources for producing DHA will facilitate the access of these nutrients
to children in Colombia
Biography
Juan Martin Delgado is a Food Engineer and did his MSc in Engineering from Universidad de Antioquia, Colombia. He is part of Bioprocess Research Group. At this mo-
ment, he is leading the development of Biotechnology applied to Food Science. He has experience as a Food Safety Consultor, as well as in the fields of alternative uses
of fish wastes, fish silage, bromatological analysis, and microalgae culture. His passion is to find new ways to use biotechnology to solve the alimentary needs of children
in Colombia.
martin.delgado@udea.edu.coJuan Martin Delgado et al., J Biotechnol Biomater 2017, 7:4 (Suppl)
DOI: 10.4172/2155-952X-C1-079