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Volume 9, Issue 11 (Suppl)

J Cancer Sci Ther

ISSN: 1948-5956 JCST, an open access journal

Asia Pacific Oncologists 2017

November 20-22, 2017

November 20-22, 2017 Melbourne, Australia

Asia Pacific

Oncologists Annual Meeting

The inactivation of tumor suppressor genes by increased H3K9me3 drives spontaneous transformation

of rat MSCs

Yong Zheng and Liu He

Wuhan University, China

odent mesenchymal stromal cells (MSCs) have been demonstrated to spontaneously undergo tumorigenic transformation

after long term

ex vivo

culture. The mechanism leading to the MSCs spontaneous transformation is unclear. To investigate

the role of H3K9me3 for the spontaneously transformation of MSCs, the pre-senescent and transformed MSCs were prepared

according to the criterion described previously. H3K9me3 target genes were evaluated with ChIP-on-chip arrays. The

expression of tumor suppressor genes (

CDKN2B, CDKN2C, CDKN1C

and

PTEN

) was evaluated with RT-qPCR, these gene-

associated H3K9me3 were quantified with chromatin immunoprecipitation (ChIP) and the DNA methylation levels were

analyzed with bisulfite DNA sequencing (BSP). We found that there were 1277 genes in pre-senescent MSCs and 2519 genes in

transformed MSCs targeted by H3K9me3 (Cutoff: FDR≤0.05). The genes associated with H3K9me3 are related to the catalogs

of cell differentiation, development and nucleotide and protein metabolism. The expression of

CDKN2B, CDKN2C, CDKN1C

and

PTEN

were obviously decrease in transformed MSCs, with an up-regulation in genes associated H3K9me3 and CpG

sites methylation. These results demonstrate that an H3K9me3 enhanced DNA methylation contributes a crucial role in the

spontaneous transformation of MSCs.

Biography

Yong Zheng is presently studying the mechanisms underlying these changes of epigenetic modification. He has his research interests in understanding the

molecular mechanisms of tumorigenesis. He has identified the key stages during the spontaneous of rat mesenchymal stem cells and his previous study identified,

for the first time, an Ezh2/H3K27me-independent and H3K9me enhanced aberrant DNA methylation of the

p16

gene, which might be an epigenetic signature for

MSC spontaneous transformation.

zhengyong@whu.edu.cn

Yong Zheng et al., J Cancer Sci Ther 2017, 9:11 (Suppl)

DOI: 10.4172/1948-5956-C1-117