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Analytica 2016
September 28-30, 2016
Volume 7, Issue 5(Suppl) J Anal Bioanal Tech 2016
ISSN: 2155-9872 JABT, an open access journal
conferenceseries .com
September 28-30, 2016 Orlando, USA
7th International Conference and Exhibition on
Analytical & Bioanalytical Techniques
Saroj Kumar et al., J Anal Bioanal Tech 2016, 7:5(Suppl)
Infrared spectroscopy combined with imaging modalities is a new technique to understand the
disease pathology
Saroj Kumar1,2 , X Liu2, F Borondics3, B Popescu4, E Goormaghtigh 5 and F Nikolajeff1
1Uppsala University, Sweden
2Canadian Light Source, Canada
3SOLEIL, France
4University of Saskatchewan, Canada
5Université Libre de Bruxelles, Belgium
Development of modern infrared spectroscopy has a wide range of biological applications. Initially, it was extensively used
for protein secondary structure analysis as well as nucleotides, lipids and carbohydrates. Now with time, it extended to
biodiagnositic tools such as cells, tissues and bio-fluids. Infrared imaging can be used to discriminate between healthy and
diseased one. IR microscope equipped with FPA (focal plane array) detector able to scan the larger area with quick time
and that helps to measures the cells as well as tissue (histopathology). An IR synchrotron light source connected with IR
microscope further enhances the spatial resolution at diffraction limit. The use of this method of infrared spectroscopy in
disease pathology with two examples (breast cancer and multiple sclerosis) will be presented in this study. The spectroscopic
imaging data on breast cancer and multiple sclerosis samples were acquired in transmission on deparaffined 3-5 μm thick tissue
slices deposited on 40×26 mm2 BaF2 slides. For cells, the fibroblasts were grown on CaF2 window and directly used for FTIR
measurements. We used a hyperion imaging system (Bruker) equipped with a 64*64 MCT (Mercury-Cadmium-Telluride)
FPA (Focal Plane Array) detector. FTIR imaging technique was used to discriminate healthy and diseased samples on the basis
of chemical changes due to its potential to probe tissues and cells at the molecular level. Now with the application of advanced
focal plane array detector, large area of samples in a short time can be scanned and investigated the specific changes that could
be correlated with the pathology and different environmental stresses.
Biography
Saroj Kumar has completed his PhD from Stockholm University, Sweden and Postdoctoral studies from Universite Libre de Bruxelles, Belgium and Canadian Light
Source, Canada. He is the Project Leader in Department of Engineering Science, Uppsala University, Sweden. He has published more than 22 papers in reputed
journals and has been serving as a Reviewer in reputed journals.