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Analytica 2016
September 28-30, 2016
Volume 7, Issue 5(Suppl) J Anal Bioanal Tech 2016
ISSN: 2155-9872 JABT, an open access journal
conferenceseries .com
September 28-30, 2016 Orlando, USA
7th International Conference and Exhibition on
Analytical & Bioanalytical Techniques
Faiz Ali et al., J Anal Bioanal Tech 2016, 7:5(Suppl)
Separation of peptides and proteins in tryptic digest of cytochrome c by novel step elution approach
in open tubular capillary electrochromatography
Faiz Ali1,2 , Won Jo Cheong2 and Behisht Ara 1
1Department of Chemistry, University of Malakand, Chakdara,Pakistan
2Department of Chemistry, Inha University, South Korea
Amulti-monomer based copolymer layer was immobilized on the inner surface of a pretreated 1.1 m long silica capillary
column (50 µm internal diameter and 1.02 m effective length) after the attachment of 4-(trifluoromethoxy)phenyl
isocayanate and sodium diethyl dithiocarbamate initiator system. The attachment of initiator system to silanol functionalities
on the inner capillary surface was assisted by dibutyl tin dichloride catalyst. The copolymer immobilized open tubular
capillary column resulted in the separation of about 40 peaks out of tryptic digest of cytochrome C sample in capillary
electrochromatography with high separation efficiency (Ca. 220,000 plates/column) for some of the peptide peaks. A novel
step elution approach was also demonstrated for the separation of tryptic digest of cytochrome C where two mobile phases
having different water content were used during the same run resulting in the separation of higher number of peptide peaks
(Ca. above 50) out of tryptic digest of cytochrome C, with much improved peak capacity. The step elution approach in addition
to the open tubular nature and increased column length could be a good strategy for proteomic analysis with enhanced peak
capacity in capillary electro-chromatography.
Biography
Faiz Ali has completed his PhD at the age of 31 years from INHAUniversity South Korea and postdoctoral studies from the same University. He is the Editorial board
member for the UK Journalof Pharmaceutical and Biosciences (UKJPB He has published about 12 papers in reputed
SCI journals and has presented about 12 conference presentation in international reputed conferences.