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Journal of Clinical & Experimental Pathology | ISSN: 2161-0681 | Volume 8
December 03-04, 2018 | Chicago, USA
American Pathology and Oncology Research
&
15
th
International Congress on
Microbial Genetics and Molecular Microbiology
International Conference on
HuR (ELAV1) as a potential tumor marker in gastroesophageal junction adenocarcinoma
Wenyi Luo
1
, Tongfeng Tan
and
Erin Rubin
2
1
Anderson Cancer Center, USA
2
University of Oklahoma Health Sciences Center, USA
H
uR is a master protein involved in the regulation of mRNA stability. Increased HuR expression and cytoplasmic translocation
in tumors are associated with poor prognoses and altered responses to chemotherapy. HuR expression has been studied in
esophageal squamous cell carcinoma, but not in gastroesophageal junction (GEJ) adenocarcinoma arising in Barrett's esophagus.
To study HuR, formalin fixed paraffin embedded tissue blocks of twenty patients who underwent endoscopic mucosal resection
for GEJ adenocarcinoma without pre-operative neoadjuvant therapy and five patients with Barrett's esophagus without dysplasia
were retrieved upon approval of Institutional Review Board. Tissue blocks were sectioned and stained with 1:500 diluted mouse
monoclonal anti-HumanHuR antibody clone HuR-Rb SC-5261 (Santa Cruz, CA) and a horseradish peroxidase-conjugated secondary
antibody with a Leica BOND-III automated IHC/ISH-stainer. The cytoplasmic and nuclear staining patterns of HuR were evaluated
separately and scored. The intensity and cytoplasmic localization of HuR staining correlate with the neoplastic potential of the lesion.
HuR staining is only detected in nuclei of benign metaplastic columnar mucosa (nuclear AIRS:2.3; cytoplasmic AIRS: 0). Barrett’s
epithelium shows stronger nuclear staining (AIRS:6.0) and some cytoplasmic staining (AIRS:5.2). Adenocarcinomas including
poorly-differentiated adenocarcinoma and adenocarcinoma with mucinous differentiation show markedly increased HuR staining
in both nuclei (AIRS:9.4) and cytoplasm (AIRS:9.4). In specimens with Barrett’s epithelium and dysplasia, HuR expression appears
higher in the latter. This study provides a potential novel diagnostic and differential diagnostic marker of esophageal glandular
neoplasms and may also provide a novel therapeutic opportunity.
luowenyi2008@hotmail.comJ Clin Exp Pathol 2018, Volume 8
DOI: 10.4172/2161-0681-C5-058