6 / 6
Page 19
Notes:
conferenceseries
.com
Volume 9
Journal of Alzheimers Disease & Parkinsonism
Alzheimer's Congress 2019
March 20-21, 2019
March 20-21, 2019 Sydney, Australia
11
th
World Congress on
Alzheimer's Disease & Dementia
Joshua Kuruvilla et al., J Alzheimers Dis Parkinsonism 2019, Volume 9
DOI: 10.4172/2161-0460-C2-063
Investigating the autophagy mechanisms of orientin in lipopolysaccharide-stimulated BV2
microglia cells
Joshua Kuruvilla, Anna Ling Pick Kiong, Kenny Voon Gah Leong, Koh Rhun Yian and Wong Ying Pei
International Medical University, Malaysia
Background & Aim:
Neuro inflammation is a primary risk factor of Neurodegenerative Diseases (ND), with microglia
cells under pathological conditions directly contributing to neuro inflammation. Induced autophagy has been known to
therapeutically reduce neuro inflammation without exacerbating the pathological condition of the disease. Existing treatments
for inducing autophagy in neuronal setting are few but effective, with some noted to have reached clinical trials phase II and
much scientific support for new compounds to modulate autophagy in a neuronal setting. Hence, this study aims to focus on
the autophagic inducing potential of orientin on lipopolysaccharides-stimulated BV2 microglial cells.
Method:
BV2 microglia cells pre-treated with orientin at maximum non-toxic dose or MNTD (15 µM) and ½ MNTD (7.5
µM), for a 3-hour period, followed by induction of neuro inflammation via 0.1 μg/mL of Lipopolysaccharide (LPS) stimulation.
Autophagolysosome production was qualitatively determined with Acridine Orange (AO) staining and expression of autophagy
pathway proteins was analyzed via Western Blot analysis.
Result:
The induction of intracellular autophagolysosomes, under MNTD and ½ MNTD treatment of orientin qualitatively
determined by AO staining confirmed the near completion of autophagy, with particular noteworthy observation of low
complete neuronal death. Western Blot results showed up regulation of autophagy proteins Beclin-1, ATG5 and LC3-II,
highlighting upregulation of key autophagy pathways in autophagy vacuole formation.
Conclusion:
Orientin possesses significant likelihood of contributing to field of autophagy inducing therapeutic agents for
targeting neuro inflammation in neurodegenerative diseases. Its autophagy inducing properties most likely stem from its
ability to directly affect the mTOR signaling pathways by down regulating PI3K-I/Akt and MAPK/Erk 1/2 signaling pathway,
encouraging future studies are required to refine this hypothesis.
Biography
Joshua Kuruvilla has completed his graduation in Medical Biotechnology in Biological Sciences.
Joshua.Kuruvilla@student.imu.edu.my