agri-world-2018-scientifictracks-abstracts

Page 55

Notes:

conferenceseries

.com

World Congress on

March 05-07, 2018 | Paris, France

Plant Biotechnology and Agriculture

Volume 6

Advances in Crop Science and Technology

ISSN: 2329-8863

Agri World 2018

March 05-07, 2018

Molecular characterization of ten

Heliconia

(Heliconiaceae) genotypes by means of RAPD markers

Moumita Malakar, Pinaki Acharyya

and

Sukanta Biswas

University of Calcutta, India

eliconia

a trendiest specialty cut flower of tropics is securing popularity for its inflorescence’s charismatic form and alluringly

blended hues in the world and in Indian market too. It is a monophyletic genus with roughly 200 to 250 species of neotropical

origin. It resides alone in

Heliconia

ceae family of Zingiberales order former incorporated in Musaceae for fusion of inverted flowers,

single staminode and drupe type fruits elite for it. Exploitation of molecular markers may aid in perceptive recognition of several

species and varieties of them since taxonomic confusions and uncertainties subsist amid them. In this context, ten species and

varieties were analyzed using RAPD markers. Chosen thirty primers among seventy, amplified 1281 polymorphic DNA fragments

with each primer giving a mean of 42.7 polymorphic bands. The genetic similarity matrix constructed with Jaccard’s coefficient

using RAPD marker scores showed that the highest value was between

H. psittacorum

var. Choconiana and

H. psittacorum

var.

Lady Di (0.384), while the lowest was between

H. psittacorum

var. Choconiana and H. stricta var. Dwarf Jamaican Red (0.244). Ten

species and varieties of

Heliconia

formed three distinct clusters at similarity coefficient value of 0.33, implying a parallelism between

genetic and morphologic or taxonomic variability of

Heliconia

genotypes. Golden Torch, Choconiana, Lady Di and

H. wagneriana

included in cluster I while Choconiana, Lady Di formed a more cohesive entity. On the contrary,

H. stricta

var. Dwarf Jamaican

Red produced a separate cluster validating that taxonomically related entries clustered together and distant ones segregated. So, the

current sequels demonstrate that RAPD is a rapid, relatively economical and functional for the characterization of genetic divergence

between different

Heliconia

genotypes.

Biography

Moumita Malakar is a Senior Research Fellow (INSPIRE Fellow) at University of Calcutta, India, pursuing Doctoral program on Horticulture, specializing in

Floriculture & Landscaping. She studied longevity of few ornamental foliages and flowers.

moumitamalakar7@gmail.com

Moumita Malakar et al., Adv Crop Sci Tech 2018, Volume 6

DOI: 10.4172/2329-8863-C1-005