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conferenceseries
.com
11
th
World Congress on
March 05-07, 2018 | Paris, France
Plant Biotechnology and Agriculture
Volume 6
Advances in Crop Science and Technology
ISSN: 2329-8863
Agri World 2018
March 05-07, 2018
Mutated and wild type
Gossypium
Universal Stress Protein-2 (GUSP-2) gene confers resistance to stresses
in
Escherichia coli
,
Pichia pastoris
and cotton plant
Muhammd Nadeem Hafeez
Center for Excellence in Molecular Biology, Pakistan
G
ossypium arboreum
is considered to be a rich source of stress responsive genes and EST data base revealed that mostly of its genes
are uncharacterized. The full length
Gossypium
universal stress protein-2 (
GUSP-2
) gene (510 bp) was cloned in
E. coli
,
Pichia
pastoris
and
Gossypium hirsutum
, characterized and point mutated at three positions separately at 352-354, Lysine-60 to proline (
M1-
usp-2
) and 214-216, aspartic acid-26 to serine (
M2-usp-2
) and 145-147, Lysine-3 to proline (
M3-usp-2
) to study its role in abiotic
stress tolerance. It was found that heterologous expression of one mutant (
M1-usp-2
) provided enhanced tolerance against salt and
osmotic stresses, recombinant cells have higher growth up to 10-5 dilution in spot assay as compared to Wusp-2 (wild type GUSP-
2),
M2-usp-2
and M3-usp-2 genes.
M1-usp-2
in Pichia pastoris transcript profiling exhibited significant expression (7.1-fold) to salt
and (9.7) and osmotic stresses.
M1-usp-2
gene was also found to enhance drought tolerance and significant expression (8.7) in CIM-
496-Gossypium hirsutum transgenic plants. However, little tolerance against heat and cold stresses both in recombinant yeast and
bacterial cells was observed. The results from our study concluded that activity of
GUSP-2
was enhanced in
M1-usp-2
but wipe out
in M2-usp-2 and M3-usp-2 response remained almost parallel to
W-usp-2
. Further, it was predicted through in silico analysis that
M1-usp-2, W-usp-2
and
M3-usp-2
may be directly involved in stress tolerance or function as signaling molecule to activate the stress
adaptive mechanism.
Biography
Muhammd Nadeem Hafeez is currently working as a Researcher at Center for Excellence in Molecular Biology (CEMB), Lahore, Pakistan. He has expertise in gene
editing, cloning and plant transformation. He has developed a first triple gene genetically engineered cotton variety of Pakistan, which confers significant resistance
against biotic and abiotic stresses.
chnadeemhfz@gmail.comMuhammd Nadeem Hafeez, Adv Crop Sci Tech 2018, Volume 6
DOI: 10.4172/2329-8863-C1-005