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Volume 4, Issue 4(Suppl)
J Infect Dis Ther 2016
ISSN: 2332-0877, JIDT an open access journal
Page 81
Notes:
Infectious Diseases 2016
August 24-26, 2016
conferenceseries
.com
August 24-26, 2016 Philadelphia, USA
&
Infectious Diseases
Joint Event on
2
nd
World Congress on
Pediatric Care & Pediatric Infectious Diseases
International Conference on
Development of nano-based microemulsion from tea tree oil and silver nanoparticles and assessment of
their enhanced antimicrobial activities against representative wound infecting pathogens
Ashraf Osman Abdellatif Mohamed
1,2
, Suad Y Alkarib
1
and
Alaa M Shawkey
2
1
Karary University, Sudan
2
Cairo University, Egypt
C
ombining and incorporating of the natural antimicrobials with metal nanoparticles is a growing research area because of their
potential applications in nanomedicines to enhance and synergize their activity as well as to minimize unwanted side effects
by using lower concentrations. The aim of the present study was to investigate the feasibility of using tea tree oil (TTO) and greenly
synthesized silver nanoparticles (AgNPs) alone and in micro emulsion combination formula against representative wound infecting
pathogens namely;
Staphylococcus aureus
, methicillin resistant
Staphylococcus aureus
(MRSA),
Pseudomonas aeruginosa
and
Candida
albicans
. The result of the bioassay showed that both TTO-AgNPs combination possesses a potential antimicrobial property. Based on
the minimum lethal concentration (MLC), the fractional lethal concentration index (FLCI) showed that treatment with combinations
of TTO and AgNPs exerted a synergistic effect against MRSA (FLCI=0.233) followed by
P. aeruginosa
(FLCI=0.263) and indifferent
effects against
S. aureus
and
C. albicans
(0.53 and 0.73, respectively). As well, analysis of the morphological changes in
S. aureus
cells
by transmission electron microscope (TEM) revealed that, AgNPs aggregated and located non-specifically on the cell wall, also,
AgNPs were seen within the cell wall or the cell of bacteria. The cells became severely distorted and disrupted showing a bactericidal
action when compared to untreated cells. In conclusion, these results showed the potential of using TTO and AgNPs in form of micro-
emulsion combination and this could be developed into a broad spectrum pharmaceutical product for treating wound infections.
Biography
Ashraf Osman Abdellatif Mohamed is currently a PhD student at Faculty of Pharmacy, Cairo University, Egypt. He has published five papers in reputed journals.
ashrafpharm@hotmail.comAshraf Osman Abdellatif Mohamed et al., J Infect Dis Ther 2016, 4:4(Suppl)
http://dx.doi.org/10.4172/2332-0877.C1.008