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Volume 4, Issue 4(Suppl) J Infect Dis Ther 2016
ISSN: 2332-0877, JIDT an open access journal
Page 79
Notes:
Infectious Diseases 2016
August 24-26, 2016
conferenceseries.com
August 24-26, 2016 Philadelphia, USA
&
Infectious DiseasesJoint Event on 2nd World Congress on
Pediatric Care & Pediatric Infectious Diseases
International Conference on
Volatile compounds in the stem bark of Sclerocarya birrea (Anacardiaceae) possess antimicrobial activity
against drug-resistant strains of Helicobacter pylori
Roland N Ndip1, 2, C Njume 1, A J Afolayan1 and E Green1
1University of Fort Hare, South Africa
2University of Buea, Cameroon
The aim of this study was to isolate and identify phytochemicals with anti-Helicobacter pylori activity from the stem bark of
Sclerocarya birrea. The plant crude extract was fractionated by silica gel column and thin layer chromatography techniques;
initially with ethyl acetate (EA) and subsequently with a combination of ethyl acetate/methanol/water (EMW). Further fractionation
and identification of the phyto-constituents was achieved by gas chromatography and mass spectrometry (GC/MS) analysis. The
antimicrobial activity of the fractions and compounds was evaluated against 5 metronidazole and clarythromycin-resistant strains of
H. pylori and a reference strain ATCC 43526 using micro-broth dilution technique. Amoxicillin was included in these experiments
as a positive control antibiotic. Sixteen of the 18 fractions collected demonstrated anti-H. pylori activity with minimum inhibitory
concentration (MIC50) values ranging from 310-2500 µg/mL. Two of the fractions; EMW fraction 6 and EA fraction 1 revealed the
presence of 5 and 24 compounds respectively representing 40.5% and 86.57% of the total composition. Most of the compounds were
essential oils with terpinen-4-ol being the most abundant agent (35.83 %), followed by pyrrolidine (32.15 %), aromadendrene (13.63%)
and α-gujunene (8.77%). MIC50 values for amoxicillin, terpinen-4-ol and pyrrolidine ranged from 0.0003-0.06 µg/mL, 0.004-0.06 µg/
mL and 0.005-6.3 µg/mL respectively. The inhibitory activities of terpinen-4-ol and pyrrolidine were similar to amoxicillin (P>0.05).
Most of these compounds are being reported in this plant for the first time and may represent new sources of therapeutically useful
compounds against H. pylori.
Biography
Roland N Ndip has obtained his PhD in 1994 from the Edo State University, Nigeria. He has worked as a Professional Microbiologist for over 20 years successively at
Edo State University, Nigeria; University of Fort Hare, South Africa and the University of Buea, Cameroon rising to the rank of Professor of Microbiology. His research has
concentrated in the areas of microbiology, molecular biology, antimicrobial chemotherapy and alternative and complementary medicine. He has published over 100 articles
in international peer reviewed journals of repute. He currently serves as a Registrar at the University of Buea, Cameroon.
Roland N Ndip et al., J Infect Dis Ther 2016, 4:4(Suppl)