Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Google Scholar citation report
Citations : 2154
Journal of Biotechnology & Biomaterials received 2154 citations as per Google Scholar report
Indexed In
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- Open J Gate
- Genamics JournalSeek
- Academic Keys
- ResearchBible
- China National Knowledge Infrastructure (CNKI)
- Access to Global Online Research in Agriculture (AGORA)
- Electronic Journals Library
- RefSeek
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Geneva Foundation for Medical Education and Research
- Euro Pub
- ICMJE
Useful Links
Recommended Journals
Related Subjects
Share This Page
In Association with
Utilization of lipase enzyme produced by Bacillus sp. as a biocatalyst in the production of biodiesel
World Bio Summit & Expo
Muhannad I Massadeh and Rana Dajani
Hashemite University, Jordan
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
Biodiesel can be produced by transesterification of oils using methanolysis in the presence of chemical or biological catalyst. Lipase enzyme produced by Bacillus sp. isolated from a raw petrol sample was evaluated for its efficiency as a catalyst in the production of biodiesel. The Bacillus spp. was identified by morphological, biochemical and physiological characteristics as Bacillus stearothermophilus. The production of lipase enzyme by B. stearothermophilus was investigated and its properties were evaluated. The enzyme exhibited moderate stability in organic solvents and seemed to be activated by isopropanol at a concentration of 25% and 100%. The enzyme retained more than 94% of its activity in a buffer system supplied with ethylenediaminetetraacetic acid (EDTA) compared to other metal ions investigated. The extracellular lipase enzyme from B. stearothermophilus was purified by ammonium sulfate precipitation by bringing it to 40% saturation, followed by ion exchange chromatography. The SDS-PAGE of the purified fraction showed two bands having a molecular mass of 35 and 50 kDa. The LC MS/MS analysis of the two bands revealed no matches when searched against the NCBRI database suggesting that this lipase enzyme is a novel protein. The lipase enzyme produced was used in the transesterification experiments to evaluate its efficiency as a biocatalyst for the production of biodiesel. Four lipase preparations were used for the esterification of palm oil: Crude lipase, pure lipase, free enzyme from immobilized B. stearothermophilus cells, and pure lipase immobilized in sodium alginate beads. Compared to chemical transesterification, the free and pure form of lipase showed moderate efficiency to convert palm oil into biodiesel. The maximum yield of biodiesel obtained was 70% when lipase from immobilized cells was employed which is considered valuable as it affects the cost of the production process throughout the direct production of biodiesel.Biography
Email: massadeh@hu.edu.jo
