Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Google Scholar citation report
Citations : 2154
Journal of Biotechnology & Biomaterials received 2154 citations as per Google Scholar report
Indexed In
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- Open J Gate
- Genamics JournalSeek
- Academic Keys
- ResearchBible
- China National Knowledge Infrastructure (CNKI)
- Access to Global Online Research in Agriculture (AGORA)
- Electronic Journals Library
- RefSeek
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Geneva Foundation for Medical Education and Research
- Euro Pub
- ICMJE
Useful Links
Recommended Journals
Related Subjects
Share This Page
In Association with
Use of 3-untranslated region of messenger RNA from measles virus matrix protein as a stabilizing cis-element for increased protein production yields
23rd International Conference on Pharmaceutical Biotechnology
Marzieh Marzbany, Fatemeh Ghassemi and Mahsa Rasekhian
Kermanshah University of Medical Sciences, IranPasteur Institute of Iran, Iran
Posters & Accepted Abstracts: J Biotechnol Biomater
Abstract
The market for the use of recombinant proteins for medical applications has been increasing in recent years. Expression vectors are one of the decisive factors in the cost-effectiveness of the production process of recombinant proteins. The genetic factors found at the 3’untranslated region of mRNA expressed by such vectors, play an important role in determining its stability and thus in the efficiency of the recombinant protein production process. We studied the effect of 3'UTR of matrix protein mRNA from measles virus on mRNA stability by a GFP-based reporter construct in three cell lines. Application of 3'UTR of matrix protein mRNA from measles virus increased the GFP-mRNA stability in a time and cell dependent manner. Analysis of the 3'UTR of matrix protein mRNA from measles virus for presence of known cis-acting motifs indicated the occurrence of two PABPC1 binding sites, known for its stability and translation enhancing effects. Our results verified the potentiality of 3'UTR region of matrix protein mRNA for improvement of recombinant protein production and vector design for mammalian cell hosts.Biography
E-mail: marzehmarzbany@yahoo.com
