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UPLC-HRMS Based Untargeted Metabolic Profiling Reveals Changes In Chickpea (cicer Arietinum) Metabolome Treated With PGR And PGPR | 106321
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UPLC-HRMS based untargeted metabolic profiling reveals changes in chickpea (cicer arietinum) metabolome treated with PGR and PGPR
Joint Event on 3rd International Conference on Ecology, Ecosystem and Conservation Biology & 3rd International Conference on Microbial Ecology & Eco Systems
Naeem Khan, Asghari Bano and Md Ali Babar
Department of Plant Sciences, Quaid-I-Azam University, Islamabad,PakistanDepartment of Biosciences, University of Wah, Wah Cantt.PakistanDepartment of Agronomy, IFAS, University of Florida, Gainesville,USA
Genetic improvement
for drought tolerance
in chickpea requires a solid
understanding of biochemical
processes involved with
different physiological
mechanisms. The objective of
this study is to demonstrate
physiological changes
and altered metabolic
levels in chickpea varieties
(tolerant and sensitive)
treated with PGPR and
PGRs and grown under
contrasting water regimes.
Ultrahigh performance
liquid chromatography-high
resolution mass spectrometry
(UPLC-HRMS) analyses
was carried out to classify
metabolites associated with
drought tolerance in chickpea.
The seeds of two chickpea
genotypes (Punjab Noor-
2009 and 93127) differing in
sensitivity to drought were
soaked for 2-3 h prior to
sowing in 24 h old cultures
of isolates. The salicylic acid
(SA) and putrescine (Put)
were sprayed (150 mg/L),
on 25 days old seedlings of
chickpea. The result showed
that plants treated with
consortium of PGPR and
PGRS significantly enhanced
the chlorophyll, protein
and sugar contents. Highly
significant increases were
recorded for relative water
content in PGPR and PGRs
treated plants. Leaf proline
content, lipid peroxidation
and activities of antioxidant
enzymes (CAT, APOX, POD
and SOD) were increased in
response to drought stress but
decreased due to PGPR. Grain
weight, number of nodules,
pod weight and total biomass
were higher in PGPR and
PGR treated plants grown in
sandy soil. Proline, L-arginine,
L-histidine, L-isoleucine and
tryptophan were accumulated
in the leaves of chickpea
exposed to drought stress.
Consortium of PGPR and
PGRs induced significant
accumulation of riboflavin,
L-asparagine, aspartate,
glycerol, nicotinamide, and
3-hydroxy-3-methyglutarate in
leaves of chickpea. Sensitive
genotype showed significant
accumulation of nicotinamide
and 4-hydroxy-methylglycine
in PGPR and PGR treated
plants at both time points (44
and 60 days) as compared
to non-inoculated drought
plants. Arginine accumulation
was also enhanced in the
leaves of sensitive genotype
under drought condition.
Metabolic changes in light
of drought condition and
in presence of PGPR and PGRs highlighted pools of
metabolites that affect the
metabolic and physiological
adjustment in chickpea that
reduced drought impacts.
Therefore, the integrative use
of consortia of PGPR and SA
could be an effective ecofriendly
approach to induce
drought tolerance in crop
plants.
Biography
Naeem Khan has his expertise in the field of Plant-Microbe Interactions and Plant Sciences. His scientific interest includes the study of Plant-Microbe Interactions, Abiotic stresses, Phytoremediation and Metabolic responses of Plants to environmental stresses. He employed a non-targeted global ultrahigh performance liquid chromatography-high resolution mass spectrometry (UPLC–HRMS) analysis to identify metabolites from the leaf tissue of irrigated and drought-stressed chickpea plants inoculated with PGPRs. He has published many papers in well reputed international journals related to plantmicrobe interactions, plant metabolites and role of PGPR in phytoremediation.
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