Journal of Biotechnology & Biomaterials
Open Access

Abstract

In order to solve the drawbacks of sensitivity and portability in optical biosensors we have developed ultrasensitive and miniaturized photonic silicon sensors able to be integrated in a �?¢�?�?�?�?lab-on-a-chip�?¢�?�?�? microsystem platform. The sensors are integrated Mach-Zehnder interferometers based on TIR optical waveguides (Si/SiO2/Si3N4) of micro/nanodimensions. We have applied this biosensor for DNA testing and for detection of single nucleotide polymorphisms at BRCA-1 gene, involved in breast cancer development, without target labeling. The hybridization of different concentrations of DNA target with two mismatching bases corresponding to a mutation of the BRCA-1 gene was detected with this biosensor. A limit of detection of 25 nM was achieved.

Biography

José Sánchez del Río Sáez is a postdoctoral researcher in Alter Technology. He was a postdoc in the Face Recognition and Artificial Vision (FRAV) group of the Statistical and Informatics department at Rey Juan Carlos University (URJC). He has worked in CSIC for 5 years in the IEM-CSIC analyzing big data collected from physics experiments at big facilities. He has written 4 patents as a patent researcher in Biometrics in an international IT company. He did his PhD in Biotechnology in the Biosensor group in Madrid at IMM-CNM-CSIC.