Journal of Clinical & Experimental Pathology
Open Access

Abstract

Canine transmissible venereal tumor (CTVT) is a commonly occurred in genital and extra-genital sites of both genders. The tumor is spread through tumor cell transplantation from affected to unaffected dogs usually during coitus. LINE-1 or L1 retrotransposons has pivotal role in allogenic transfection amongst uncontrolled dog population. In the study, it is aimed to evaluate macroscopical, cytological and histopathological findings of canine transmissible venereal tumor in transfected dogs during chemotherapy. Immunohistochemically, the tumor was also investigated in which phase was found using specific markers (CD3, CD4, CD8, CD79, TGF-β) and amplified specific sequence of TVT LINE (LINE-1) retrotransposon by in situ PCR method. Polyhedral shaped neoplastic cells which had large, round, hypo/hyper-chromatic nuclei and eosinophilic cytoplasm were detected. All markers were found positively in especially early weeks of recovery. CD4 and TGF-β markers were also conspicuously positive at initial stage. In situ PCR, LINE-1 sequence was initially positive in only 4 cases. It is believed that CDs and TGF- β provided phase identification of tumor at initial and during chemotherapy. And, it is thought that presence of T and B lymphocytes, which have roles in cellular and humoral immunity, need so that regression of the tumor is possible.

Biography

Sevil Atalay Vural completed her PhD thesis (Comparison of Histopathological, Immunoperoxidase and Immunofloresan Methods in Diagnosis of Rabies Virus Infection in Dogs) degree from the Ankara University Faculty of Veterinary Medicine, Department of Pathology in 1997. She was assigned as Professor in 2008 and she is currently at this University. Her main research areas are nervous system and genital system disorders, immunohistochemical and molecular diagnosis including IS-PCR and ISH of tumours, apoptotical mechanism in different natural and experimental animal models. She has conducted and worked 21 scientific projects. Her articles over than 65 have been published in well known international journals. Also, she has presented over than 60 posters and oral presentations in national and international congress.