Journal of Gastrointestinal & Digestive System
Open Access

Abstract

Statement of the Problem: Helicobacter pylori (H. pylori) antibiotics resistance is the major cause of failure in eradicating H. pylori. Recent studies have showed that the virulence genes associated with H. pylori antibiotics resistance have been isolated from adult peptic ulcer patients. To date, however, there is still lack of evidene about this in Vietnamese pediatric children. The objective of this study is to evaluate the association between H. pylori antibiotics resistance and the presence of cagA and vacA genes in pediatric patients. Methodology & Theoretical Orientation: 150 samples of H. pylori isolated from 150 infected pediatric patients whose antimicrobial susceptibility showed a resistance to at least 1 of 3 antibiotics: amoxicillin, clarithromycin and metronidazole. The cagA and vacA genes were detected by using multiplex PCR (Polymerase Chain Reaction), between January, 2012 through September, 2013 in National Hospital of Pediatrics, Hanoi, Vietnam. Findings: The result showed that the cagA gene was detected in 34% H. pylori strains. The rate of vacAs1, vacAs2, vacAm1, vacAm2, vacAs1/m1, vacAs1/m2 and vacAs2/m2 were 39.3%, 14.7%, 37.3%, 23.3%, 16.7%, 11.3% and 6.6% respectiverly. 8% strains harboured three virulence genes cagA, vacAm1 and vacAs1. Conclusion & Significance: The prevalence of cagA and the vacA alleles distribution didn�?¢�?�?�?�?t show a significant difference between susceptible and antibiotics resistance groups. Recommendations therefore, it may not be necessary to administrate different treatment regimens for children who are infected by antibiotics resistance of H. pylori with cagA, vacA positive.

Biography

Thi-Ut Nguyen is a Pediatrician. She has been working as Gastroenterologist in Gastroenterology Department of the National Pediatric Hospital. She has conducted several studies on Helicobacter pylori antibiotics resistance of gastritis and peptic ulcer among children.