Toxicology: Open Access
Open Access

Abstract

Statement of the Problem: It is known that vegetal extracts can generate positive responses in stages of several patho-processes. The anti-oxidant; protection against DNA damage and cancer activities of Coriandrum sativum were intensely studied in the last decade. Our research envisaged the polyphenolic compounds��? structure and the anti-proliferative biologic activity of C. sativum 10% alcoholic extract against HCT-116 and MCF-7 cell lines. Methodology: The C. sativum extract was obtained respecting the Romanian Pharmacopoeia 10th edition, the plant being lyophilized (with Ilshin Kryptonstraat 11, 6718WR EBE lyophilisator to -55 oC, 5 mTorr pressure and 24 hours lyophilization time). The polyphenols were determined by LC-MS and the in vitro evaluation effects by the MTT proliferation test, using HCT116 (colorectal carcinoma) and MCF7 (mammary adenocarcinoma). The cells were seed as: 2�?�?104 (MCF7) and 1�?�?104 (HCT) in 96 well plates. The lyophilized extracts were suspended in specific culture medium being obtained a 300 mg/mL C. sativum stock solution. From this, different test concentrations were prepared by dilution (300, 200, 100 and respectively 50 mcg/mL). Result & Conclusion: As a following, after 24 hours from the exposure, using HCT-116 and MCF-7 cell lines it was observed that the cellular proliferation reduced, this being correlated to dose and the alterations of cell morphology to the groups studied; to great extract doses, apoptotic and necrotic alterations were observed, both for HCT and MCF cells; the IC50, representing concentration to which a marker substance is reducing the tissues viability with 50% after a fixed time exposure period wasn��?t observed for the cell lines used in this test; the chromatographic analysis of C. sativum alcoholic extract evidenced the presence of the polyphenolic compounds, the greatest concentrations were ascertained for epicatechin (77.083 mcg/mL) and rutin (30.279 mcg/mL), substances with known hard anti-oxidant proprieties. References 1. Rayar A, Manivannan R (2015) In Vitro Cytotoxicity Activity of Phytochemicals Isolated from Coriandrum sativum Linn. in Selected Cell Lines. Journal of Pharmacy and Biological Sciences; 10(3): 38-48. 2. AL Snafi A E (2014) The Pharmacological importance and chemical constituents of Arctium lappa: A review. International Journal for Pharmaceutical Research Scholars; 3(1-1): 663-670. 3. Kumar R S, Balasubramanian P, Govindaraj P, Krishnaveni T (2014) Preliminary studies on phytochemicals and antimicrobial activity of solvent extracts of Coriandrum sativum L. roots (Coriander). Journal of Pharmacognosy and Phytochemistry; 2(6): 74-78. 4. Nithya T G, Sumalatha D (2014) Evaluation of in vitro anti-oxidant and anticancer activity of Coriandrum sativum against human colon cancer HT-29 cell lines. International Journal of Pharmacology and Pharmacy Science; 6(2): 421-424. 5. Tang E L, Rajarajeswaran J, Fung S Y, Kanthimathi M S (2013) Antioxidant activity of Coriandrum sativum and protection against DNA damage and cancer cell migration. BMC Complementary and Alternative Medicine; 13: 347.

Biography

Eugenia Dumitrescu has her expertise in the veterinary field, reproductive toxicology, heavy metals, phyto-therapy and oxidative stress in animals. She is an Associate Professor at the Faculty of Veterinary Medicine at Banat’s University of Agriculture and Veterinary Medicine, Romania.
Email:cris_tinab@yahoo.com