Journal of Infectious Diseases & Therapy
Open Access

Abstract

Due to the increasing problem of resistance to traditional antibiotics, antimicrobial peptides (AMPs) have a huge potential
as new therapeutics against infectious diseases as they are less prone to induce resistance due to their fast and non-specific
mechanisms of action. The delivery strategies based on nanotechnology has the potential to improve the efficiency and stability
of AMPs in clinical development. Moreover, nanocarriers were particularly promising for peptide delivery, controlled release
strategies and technologies against proteolytic degradation of peptides. The Polymyxin B is a well-known antimicrobial peptide
and was used as model peptide in our study. Lipid nanocapsules (LNCs) are a new generation of biomimetic nanocarriers and
were used to deliver the peptide. The aim of the present study was to produce the LNCs with antibacterial activity. We have
developed peptide-loaded reverse micelles and incorporated in LNCs by phase inversion process. In order to evaluate the
antimicrobial activity, the minimum inhibitory concentration (MIC) was determined via a broth micro-dilution method. The
activity of Polymyxin B solution and Polymyxin B-loaded LNCs were studied against the following bacterial Gram-negative
strains: Pseudomonas aeruginosa (reference strains), Pseudomonas aeruginosa (clinical strains), Escherichia coli (reference
strains), Acinetobacter baumannii AYE (reference strains). Polymyxin B was efficiency encapsulated in LNCs using reverse
micelles and the antimicrobial activity was intact. The study shows that LNCs are an excellent candidate to deliver AMPs.

Biography

Groo Anne-Claire studied Pharmacy at the University of Reims in France and then, formulation of colloidal systems at the University of Paris XI in France. She
received her Ph.D. in Pharmaceutical Sciences from university of Angers, France, specialising in the nanocarrier optimization for oral delivery in 2013. Her main
research interests during her thesis are the development and the evaluation of anticancer drug nanoparticles, for crossing the mucus layer and for improving oral
bioavailability. She is a post-doctoral researcher and develops nanocarriers to encapsulated antimicrobial peptides to treat bacterial infection diseases.