Journal of Biotechnology & Biomaterials
Open Access

Abstract

Acknowledging the need to develop rapid and sensitive bacterial recognition approaches, we functionalized the tricyanofuran hydrazone molecular switch. Of significant interest in relation to the synthesized hydrazones is the formation of two different conjugated structures upon exposure to different pH values. Many bacteria release ammonia gas, which alkalizes environments. Herein we report the synthesis of a tricyanofuran hydrazone having the function of a colorimetric pH sensor. The UV-visible absorption and fluorescence spectra exhibit reversible color changes of the tricyanofuran hydrazone solution in acetonitrile under acid-base conditions. Our results indicate that the tricyanofuran hydrazone probe can identify the bacterial targets quickly with high sensitivity. The infected samples exhibit a significant color change from orange to blue and in the mean time there is a decrease in fluorescence emission as a function of ammonia and volatile amines released from bacterial metabolites. This tricyanofuran hydrazone chromophore is proposed for use in food packaging with a pH-sensing capability.

Biography

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