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In Association with
Symbiotic nitrogen fixation of melilot in Northern Kazakhstan
12th Euro Biotechnology Congress
Galiya Akhmetova and Galina Churkina
A.I. Barayev Scientific Production Centre for Grain Farming, Kazakhstan
Posters & Accepted Abstracts: J Biotechnol Biomater
Abstract
It is known that deficiency of nitrogen in the soil can to a large extent be compensated for by biological means, from stocks of nitrogen in the soil which have been accumulated by nitrogen-fixing microorganisms. The symbiotic nitrogen fixation is done by strains of bacteria of the genus Rhizobium, isolated from nodules of melilot and genetic identification of bacteria was conducted by 16S RNA. On the basis of the selected bacteria strains inoculum was created for the pre-seed treatment of 20 samples of melilot. For this purpose liquid yeast extract was placed in a flask with biomass of the genus bacteria Rhizobium and incubated on a shaker at 24 �°C until the optimal titer. Research on the study of nitrogen fixation was conducted on southern black carbonate soil in the period from 2015 to 2016 at the stationary field A.I. Barayev SPCGF. In the flowering phase of different varieties samples of Melilotus officinalis (L.) Pall., and Melilotus wolgicus Poir., 10 plants with inoculation and without inoculation (control) were identified from each plot and the numbers of nodules formed were counted. Calculations of symbiotic nitrogen fixation were carried out by comparison with the non-legume crop. As a result of these studies, it was found that the highest nitrogen fixation was in crops of Melilotus officinalis (L.) Pall., (from inoculation) in 5 samples KD-1824, KD-1825, KD-1683, KD-1683a and KD-1823. The controls exceeded an average of 1.2-3%. In crops of Melilotus wolgicus Poir., a high nitrogen fixation in comparison to the control (no inoculation) was observed in the samples: KD-1687 (92.3%) and KD-1823 (88.9%). In other cases, molecular nitrogen fixation was negligible. The most promising examples of the Melilotus wolgicus Poir., are KD-1687, KD-1823 and Melilotus officinalis (L.) Pall., KD-1824, KD-1683, KD-1683a with the most symbiotic potential, which will be studied for further research in the selection process.Biography
Email: galiya87@hotmail.com
