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Sub-2 �¼m C18 bound silica monolith particles as HPLC stationary phase of high separation efficiency
7th International Conference and Exhibition on Analytical & Bioanalytical Techniques
Ashraf Ali Khan and Won Jo Cheong
Inha University, South Korea
Posters & Accepted Abstracts: J Anal Bioanal Tech
Abstract
Sub-2 �¼m porous silica monolith particles have been prepared successfully by sol-gel process followed by grinding and calcinations at 550 oC. The particles were derivatized with a C18 ligand followed by end-capping with a mixture of hexamethyldisilazane (HMDS) and trimethylchlorosilane (TMCS). The resultant phase was packed in glass lined stainless steel microcolumn is much better than that of commercial C-18 phase. This phase has shown some encouraging possibility for fast analysis when packed in a short column. This study offers a promising vision towards commercialization of chromatographic phases based on silica monolith particles.Biography
Email: ashrafaliswati@gmail.com
