Journal of Biotechnology & Biomaterials
Open Access

Abstract

The study comprises of a total 125 Escherichia coli strains serially isolated from patients of urinary tract infection. Out of these; 78 (62.4%) were from female, 35 (28%) from male while 12 (9.6%) from girl children. These strains were identified using Vitek 2 automated identification system (bioMeriux). Hemolysin, an important virulence marker was studied by using sheep RBC�s. Hemolysin production is seen in 18 (14.4%) isolates. The drug resistance was determined by Kirbey Baur disc diffusion method, the drug sensitivity profile revealed that the sensitivity was excellent to Imipenem and Meropenem (99.02% each) followed by Amikacin Nitrofurotoin and Levofloxacin (94.4% each. It was fair to Gentamycin (84.4%) and Ceftazidime (80%) while sensitivity was poor to tetracycllin (68.5%), Cefixime (44.8%), Cefuroxime (44%), Ciprofloxacin (43.2%), Amoxycillin+Clavulanic acid (32.5%), Ceftriaxzone (37.3%), Co- trimaxazole (28.8%) and Ampicillin (28.8%). Extended spectrum beta lactamase (ESBL), an important mechanism by which organism confers resistance to Penicillin and cephalosporin�s groups of antibiotics were detected by double disc synergy. ESBL production was seen in 34 (27.2%) strains indicating their high potential to develop multi drug resistance. The finding suggests that UTI is common in female. The E. coli are resistant to many antibiotics. The strains are capable of producing virulence marker i.e., hemolysin as well as the production of ESBL which precludes the use of penicillin and cephalosporin�s group of drugs must be tested for.

Biography

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