Journal of Gastrointestinal & Digestive System
Open Access

Abstract

Group A rotaviruses (RVA) are the main cause of neonatal calve diarrhea (NCD) in Morocco. Until now, no reports are available regarding isolation and cultivation of RVA in clinical samples from Moroccan domestic animals or children with acute gastroenteritis (AGE) and burden due to this infection in animals remain unknown. Hence, this study aims to isolate RVA strains from NCD clinical samples in order to support RVA disease control in Morocco. This isolation process constitutes a first step toward vaccine development. Thirteen fecal samples were obtained from calves with a single episode of neonate calf diarrhea at three different dairies and two samples were collected from field during a severe NCD outbreak. Diagnosis of RVA infection was based on fecal immune-chromatographic rapid test and further evaluated for their hemagglutination (HA) activity. RVA isolation was carried out on MA104 cells after inoculates were treated with different concentrations of trypsin TPCK. All RVA isolates were confirmed by LSI VetMAX� Triplex Ruminant Rotavirus and Coronavirus Real-Time PCR kit. RVA isolation was achieved for nine clinical samples following one or two passages (60%) and was properly depended on HA activity and trypsin treatment of inoculates. The first sign of CPE detected consisted of increased cell granularity, obscure cell boundaries, cell rounding, and eventual degeneration and detachment of cells. These results constitute a crucial and a necessary step allowing preventive and veterinary medicine to support RVA disease controls in the country.

Biography

Email: ennima.imane@gmail.com