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L Sanjos�1, H Crespo1, I Glaria1, L de Pablo, E Berriatua, B Sanzberro, A Eciolaza, J Eguiluz, F J Beldr�n, J L Garrido, Marta Gil, D de Andr�s, B Amorena
and R Reina
Small Ruminant Lentiviruses (SRLV) cross the species barrier, infecting ovine and caprine species and causing different
degrees of losses in the small ruminant industry. Lentiviruses crossing the barrier, from domesticated to wild-life species
or vice versa, might adapt to the new host increasing their virulence. This study represents a first step in determining red deer
susceptibility to SRLV infection, as this species might constitute an important reservoir.Blood samples from deer of different
Northern Spain areas were obtained for serological and PCR determinations. Anti-SRLV antibodies were found by different
standard and home-made ELISAs, slightly modified in order to detect deer IgG. However, evidence of infection could not
be confirmed by different PCRs covering SRLV and BIV sequences. In an effort to demonstrate the presence of virus and/or
possible virus blockade, in-vitro studies were performed employing red deer skin fibroblasts (SF). Entry assays using CAEV-
AP virionspseudotyped with envelopes from SRLV strains showed the ability of SRLV to enter SF, with significant strain
differences. Experimental infection with different SRLV strains was attempted and the cytopathic effect, RT activity in culture
supernatants and presence of viral DNA (by PCR) assessed at 16 h, day 7 and day 10. Provirus was detected; however, no RT
activity was found, suggesting low or no production of viral particles.These data provide evidence for a partial susceptibility to
SRLV infections at cell and host (
Cervidae
) levels and strongly suggest the existence, in deer, of effective restriction mechanisms
against SRLV production
in vitro
and
in vivo
Biography
L Sanjos� is recipient of a pre-doctoral fellowship of the Spanish Ministry of Economy and Competitiveness (FPI) at the Institute of Agrobiotechnology(CSIC-UPNA-
Government of Navarre). Her research on animal health is carried out in the fields of immunology, genetics and virology. Her thesisentitled ?Innate and acquired
immunity, pathogenic mechanism and epidemiological factors in infections by small ruminant lentiviruses(SRLV)? involves the study of parameters of acquired
immune response, restriction factors and the development of new diagnostic techniques. She (Zaragoza, 1985) graduated in Veterinary Sciences, obtained her
Master?s degree in Veterinary Sciences Research at theComplutenseUniversity of Madrid (Madrid, Spain) and has different Congress contributions. Presently, she
is working in the experimental phase of her pre-doctoral period, being enrolled at the Public University of Navarre doctoral study program.
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