Journal of Biotechnology & Biomaterials
Open Access

Abstract

Egg yolk-Tris is most commonly used semen extender; however, its use involves hygienic risk, interference with fertility and poor microscopic examination. Th erefore, replacement of egg yolk with a plant-based component with protective eff ects on spermatozoa would be advantageous. In the present study, semen was collected from fi ve Murrah buff alo bulls and frozen thawed in extender with diff erent concentrations of soybean lecithin (10, 15, 20 and 25%) and 20% egg yolk. Semen parameters including sperm motion characteristics (the percentage of total motile sperm and motility), plasma membrane integrity and acrosome integrity were assessed with bright fi eld microscopy, hypo osmotic swelling test (HOST) and fl uorescein isothiocyanate conjugated peanut agglutinin (FITC-PNA) method, respectively. Th e result best result was obtained for the extender supplemented with 25% soybean lecithin, with values of 62.1% for the percentage of total motile sperm (TM%), 43.4% for motility, 47.6% for plasma membrane integrity and 62.9% for acrosome integrity. TM%, motility, acrosome integrity and plasma membrane integrity in the extender containing 25% soybean lecithin were signifi cantly higher than that of other concentrations of soybean lecithin and 20% egg yolk (p < 0.05). However, the percentages of TM, acrosome integrity and plasma membrane integrity decreased with the increasing concentration of soybean lecithin in extender. In summary, the eff ect of soybean lecithin on spermatozoa quality was superior and the eff ective concentration of soybean lecithin in extender was 26% (w/v). Soybean lecithin might replace egg yolk in extender in the cryopreservation of buff alo semen

Biography

Ms Gundeep Kaur has done her post-graduation in Animal Biochemistry from National Dairy Research Institute, Karnal, Haryana, India. She completed her B.Sc Degree in Biotechnology with distinction from Kurukshetra University, Kurukshetra and was awarded the “Roll of Honor” for her outstanding achievements during course of Graduation. She has consistent academic record with several distinctions. She has been selected as Junior Research Fellow by Indian Council of Agricultural Research. She has published eleven abstracts in International conferences and has delivered several oral presentations in International and National Conferences.