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Some parameters of partially purified superoxide dismutase isolated from Fenugreek (Trigonella foenum-graecum): A therapeutic enzyme
6th World Congress on Biotechnology
Nameet Kaur1, B K Malik1 and Nidhee Chaudhary2
1Sharda University, India 2Amity University, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
Superoxide dismutase (SOD) is the primary antioxidant enzyme that repairs cells by detoxifying the damage caused by superoxide, the most common free radical in the body. It has great potential to fight against diseases related to oxidative stress and aging viz., Alzheimer�s disease, Parkinson�s disease, Diabetes, rheumatoid arthritis, neurological disorders and cancer. In the present study, SOD enzyme isolated from Fenugreek (Trigonella foenum-graecum) was found to have high specific activity of SOD reaching to 20.1 units/mg. SOD isolated was partially purified into three fractions (0-30%, 30-60% and 60-90%) using ammonium sulfate as precipitating agent, giving 0-30% fraction with highest SOD specific activity (35.5 units/mg) and was further characterized with respect to few parameters. The optimum pH and temperature conditions for maximum enzyme activity were found to be 6.0 and 50o C. The enzyme was found to be thermally stable and retained its activity up to 70o C showing its great applicability in industries with high economical feasibility. From the results obtained, it may be concluded that spice like Fenugreek (Trigonella foenum-graecum) serves as potential source of SOD, a powerful antioxidant enzyme which has immense importance in various bio-industries.Biography
Email: nameetmisha@gmail.com
