Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Journal of Ecosystem & Ecography peer review process verified at publons
Indexed In
  • CAS Source Index (CASSI)
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Online Access to Research in the Environment (OARE)
  • Open J Gate
  • Genamics JournalSeek
  • Ulrich's Periodicals Directory
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page

Single-cell analysis reveals gene-expression heterogeneity in microbial syntrophic dual-culture

4th International Conference on Biodiversity

Weiwen Zhang

ScientificTracks Abstracts: J Ecosys Ecograph

DOI: 10.4172/2157-7625.S1.020

Abstract
Microbial syntrophic metabolism has been well accepted as the heart of how methanogenic and other anaerobic microbial communities function. In this work, we applied a single-cell RT-qPCR approach to reveal gene-expression heterogeneity in a model syntrophic system of Desulfovibrio vulgaris and Methanosarcina barkeri, as compared with the D. vulgaris monoculture. Using the optimized primers and single-cell analytical protocol, we quantitatively determine geneexpression levels of 6 selected target genes in each of the 120 single cells of D. vulgaris isolated from its monoculture and dualculture with M. barkeri. The results demonstrated very significant cell-to-cell gene-expression heterogeneity for the selected D. vulgaris genes in both the monoculture and the syntrophic dual-culture. Interestingly, no obvious increase in gene-expression heterogeneity for the selected genes was observed for the syntrophic dual-culture when compared with its monoculture, although the community structure and cell-cell interactions have become more complicated in the syntrophic dual-culture. In addition, the single-cell RT-qPCR analysis also provided further evidence that the gene cluster (DVU0148-DVU0150) may be involved syntrophic metabolism between D. vulgaris and M. barkeri. Finally, the study validated that single-cell RT-qPCR analysis could be a valuable tool in deciphering gene functions and metabolism in mixed-cultured microbial communities.
Biography
Weiwen Zhang is a Professor of Microbiology and Biochemical Engineering, Head of Laboratory of Synthetic Microbiology in the School of Chemical Engineering and Technology of Tianjin University in China. Prior to joining Tianjin University, he was a Faculty with Biodesign Institute and the School of Life Science of Arizona State University for 2007-2011, and a Senior Scientist at Microbiology Department with the Pacific Northwest National Laboratory of the U.S. Department of Energy (DOE) for 2002-2007. He has broad research experience in microbial genetics, physiology and synthetic biology, has authored more than 130 peer-reviewed scientific papers. His recent research focuses on synthetic biology of photosynthetic microorganisms and analysis of microbial consortia.
Relevant Topics

http://sacs17.amberton.edu/

Top