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Simultaneous capillary zone electrophoretic determination of hydrochlorothiazide and certain angiotensin-II receptor antagonists in pharmaceutical preparations
6th International Conference and Exhibition on Analytical & Bioanalytical Techniques
Sema Koyut�¼rk and Arin G�¼l Dal
Anadolu University, Turkey
Posters-Accepted Abstracts: J Anal Bioanal Tech
Abstract
Angiotensin-II receptor antagonists along with diuretics are mainly used in the treatment of hypertension. In this study, a capillary zone electrophoretic method was developed and validated for the determination of hydrochlorothiazide, irbesartan, telmisartan and valsartan with DAD. A 40 cm effective length (48.5 cm total, 50 �¼m i.d.) fused silica capillary was used to separate the compounds. The run buffer was 15 mM borate buffer containing 10% methanol (pH 9.0). The samples were injected for 10 s under 50 mbar pressure and the applied potential was 25 kV. Under these optimum conditions, total run time was less than 7 minutes. The method was validated for linearity, precision, accuracy, specificity, stability and sensitivity. The application of the method was successfully demonstrated for individual and combined tablets of the drugs.Biography
Sema Koyutürk graduated from Adnan Menderes University, Faculty of Sciences, Department of Chemistry in 2010. She has got her MSc degree on Analytical Chemistry in 2013. She is now a PhD student at Anadolu University, Institute of Health Sciences.
Email: skoyuturk@anadolu.edu.tr
