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Sequence and recombination analysis of the AC1 gene of Mungbean yellow mosaic virus isolates

6th World Congress on Biotechnology

Meenakshi Sindhu and P K Jaiwal

Maharshi Dayanand University, India

Posters-Accepted Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.C1.044

Abstract
Mungbean yellow mosaic virus belongs to the family Geminiviridae possessing bipartite genome. It is a menace in legume production being responsible for yellow mosaic disease transmitted by the whitefly Bemisia tabaci. The DNA ��?A��? component encodes for the information related to viral DNA replication, transcription and encapsidation, whereas DNA ��?B��? encodes for movement proteins which are responsible for virus translocation. As AC1 gene codes for a replication-associated protein (Rep) which is essential for replication of virus, it is an important target for various strategies to develop virus resistance in legumes. Evolutionary relationship among AC1 genes of different MYMV isolates was studied using MEGA6. Phylogenetic trees obtained from different methods gave different results revealing a pattern in diversity among these isolates. Regions of recombination in the Rep gene of different MYMV isolates were detected using Recombination Detection Program (RDP4). There are greater chances for a recombinant virus to emerge by increasing host range and to provide sources of variation having unpredictable effects on the viral pathogenicity.
Biography

Meenakshi Sindhu is currently pursuing her PhD from Maharshi Dayanand University under the supervision of Dr. P K Jaiwal. She has been awarded DST-INSPIRE JRF by DST, New Delhi and currently working as DST-INSPIRE SRF fellow. She has a publication from her Master’s dissertation work and she is member of reputed scientific societies so as to excel her interdisciplinary research interests as well.

Email: meenakshisindhu23@gmail.com

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