Journal of Analytical & Bioanalytical Techniques
Open Access

Abstract

Five phage clones that selectively bind to a neonicotinoid insecticide imidacloprid have been selected from a commercialized phage display library containing linear 7-mer randomized amino acid residues. A competitive enzyme- linked immunosorbent assay (ELISA) for imidacloprid was developed by using a clone L7-1 displaying peptides specific to the analyte. The half-maximum signal inhibition concentration (IC50) and the limit of detection (LOD) of the phage ELISA for imidacloprid were 82 ng mL-1 and 5.6 ng mL-1, respectively. This phage ELISA showed little cross-reactivities with the compounds structurally related to imidacloprid (<1%). The average recoveries of the phage ELISA for imidacloprid in soil and water samples were in a range of 74-96% and 82-91%, respectively. The simple phage-displayed peptide technology described here has been proven to be a convenient and efficient method for the development of an alternative format of ELISA for small molecules.

Biography

Ting Xu got his PhD in ecology from the China Agricultural University (CAU) in 2003 and since then he has been working at CAU as a faculty member. Now he is a full professor and he focuses his research interests on the development of immunochemistry technologies for food safety, environmental pollution and ecological toxicology. He has co-authored 4 chapters of books and more than 50 papers published in international journals. He holds 5 Chinese patents.