Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Google Scholar citation report
Citations : 3330
Journal of Biotechnology & Biomaterials received 3330 citations as per Google Scholar report
Indexed In
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- Open J Gate
- Genamics JournalSeek
- Academic Keys
- ResearchBible
- China National Knowledge Infrastructure (CNKI)
- Access to Global Online Research in Agriculture (AGORA)
- Electronic Journals Library
- RefSeek
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Geneva Foundation for Medical Education and Research
- Euro Pub
- ICMJE
Useful Links
Recommended Journals
Related Subjects
Share This Page
In Association with
Selection of developmentally competent ovine oocytes by Brilliant Cresyl Blue (BCB) staining improves blastocyst development rate
6th World Congress on Biotechnology
Sukanta Mondal, A Mor, I J Reddy, S Nandi and P S P Gupta
National Institute of Animal Nutrition and Physiology, India
ScientificTracks Abstracts: J Biotechnol Biomater
Abstract
The brilliant cresyl blue (BCB) staining evaluates the activity of glucose-6-phosphate dehydrogenase (G6PDH); the activity of this enzyme is greatest in growing oocytes, but it declines as oocytes mature. The objective of our study was to increase the efficiency of sheep blastocyst production after in vitro maturation/fertilization (IVM/IVF) by oocyte selection before maturation. Cumulus oocytes complexes (COCs) were aspirated from surface follicles (2-6 mm diameter) of sheep ovaries in aspiration media [TCM-199, Dulbeccoâ��s phosphate-buffered saline (PBS), 0.3% BSA, heparin (10 �¼g per ml) and gentamicin (10 �¼ per ml). Only oocytes having more than 5 layers of cumulus cells and granular homogenous ooplasm were exposed to BCB stain diluted in DPBS (DPBS with 0.4% BSA) for 90 min at 38.5o C in a humidified air atmosphere; those with or without blue coloration of the cytoplasm were designated as BCB+ or BCB-, respectively. The oocytes were exposed to 13, 26 and 39 �¼M BCB. The maturation and cleavage rate of 13 �¼M (n=103), 26 �¼M (n=125) and 39 �¼M (n=97) BCB+ sheep oocytes was higher than those in 13 �¼M (n=103), 26 �¼M (n=125) and 39 �¼M (n=97) BCB-oocytes and control group (n=92). The blastocyst development rate of 26 and 39 �¼M BCB+ oocytes was significantly (P<0.05) higher than those in 26 and 39 �¼M BCB- oocytes suggesting that the staining of sheep cumulus oocyte complexes with BCB before in vitro maturation may be used to select developmentally competent oocytes for IVF.Biography
Sukanta Mondal has completed his PhD in 2006 from Indian Veterinary Research Institute, Bareilly and Postdoctoral studies from Laval University, Canada in 2010. He is working as Principal Scientist at NIANP, Bangalore. He has done pioneering and path breaking contributions in reproductive genomics of buffalo and sheep. He has published over 45 papers in various national and international journals of repute and delivered invited lectures in conferences throughout the country. He is the Associate Editor of journals viz., American Journal of Biochemistry and Molecular Biology, Asian Journal of Biotechnology, Asian Journal of Cell Biology, Current Research in Poultry Science, Biotechnology etc.
Email: sukanta781@gmail.com
