Journal of Biotechnology & Biomaterials
Open Access

Abstract

The world is fully enriched with flora varietals which has most of the therapeutic and medicinal values. People in the world consume most of the flora in the form of fruits and vegetables. Commonly consumed fruits were taken and investigated for their antioxidant activity. Antioxidants have been suggested to have a well-defined role as preservatives and preserve from getting deteriorated, rancidity or discolouration caused by oxidation. Lipid peroxidation is an important deteriorative reaction of foods which cause adverse effects such as carcinogenesis, cell DNA mutagenesis and aging. Preliminary phytochemical analysis of plant extracts showed the presence of phenols flavonoids, terpenoids and saponins. Commonly used plant species for the activity are Cucurbita maxima, Benincasa hispida, Cucumis melo, Carica papaya, Citrullus lanatus, Citrus sinensis. Quantitative estimation of flavonoids and total phenolics showed Citrus sinensis has highest phenolics content and Carica papaya has highest flavonoids content in the above samples. Antioxidant activity was evaluated by 4 types of non-enzymatic methods like Total antioxidant capacity, DPPH method, FRAP method and Modified FRAP assay. Among the plant species the ethanol extracts of Citrus sinensis has highest total antioxidant capacity. In DPPH free radical scavenging assay Citrus sinensis has highest free scavenging activity in concentration range of (50-250) μg/ml. ABTS cation scavenging assay showed Citrullus lanatus has highest ABTS cation radical scavenging activity in the concentration range of (50-250) μg/ml. In FRAP assay Citrullus lanatus has highest antioxidant activity at A 700 in the concentration range of 100 μg/ml. Modified FRAP assay showed Citrus sinensis has highest antioxidant activity at A 750 in the concentration range of 100 μg/ml.

Biography

A. Manojkumar currently doing his final year in M.Tech Biotechnology completed his B.Tech Biotechnology in Mepco Schlenk Engineering College, Sivakasi. He has been doing his post-graduation project under the supervision of Dr