Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Google Scholar citation report
Citations : 2154
Journal of Biotechnology & Biomaterials received 2154 citations as per Google Scholar report
Indexed In
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- Open J Gate
- Genamics JournalSeek
- Academic Keys
- ResearchBible
- China National Knowledge Infrastructure (CNKI)
- Access to Global Online Research in Agriculture (AGORA)
- Electronic Journals Library
- RefSeek
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Geneva Foundation for Medical Education and Research
- Euro Pub
- ICMJE
Useful Links
Recommended Journals
Related Subjects
Share This Page
In Association with
Repetitive DNA unwinding mode of the HIM-6 RecQ helicase
19th World Congress on Biotechnology
Byungchan Ahn, Seoyun Choi and Hajin Kim
University of Ulsan, South Korea Ulsan National Institute of Science and Technology, South Korea
Posters & Accepted Abstracts: J Biotechnol Biomater
Abstract
Bloom��?s syndrome (BS) is caused by mutations in the BLM gene encoding the BLM helicase. The BLM protein contains a conserved RecQ helicase domain and unwinds various DNA substrates including replication forks. Cells from BS patients lack the BLM protein show defects in the response to replicative stress and contain a multitude of chromosomal aberrations. A BLM homolog (HIM-6) has been identified in C. elegans based on its close homology to human BLM. Here, we used singlemolecule FRET technology to observe DNA unwinding in real time. Our results show that the HIM-6 repetitively unwound a long forked DNA depending on ATP concentration and resolved flap and D-loop DNA, all of which are intermediates in DNA repair. Also, we found that HIM-6 unwound at most 25 base pairs of duplex DNA in a speed of 28 nucleotides per second before returning. Besides, repetitive unwinding mode of HIM-6 was changed to unidirectional unwinding in the presence of RPA, indicating that its unwinding mode can be modulated by partner proteins in vivo. This study can be used to search proteins which would modulate a HIM-6 unwinding mode on different DNA structures such as Holliday junction and G4-quadruplex.Biography
Byungchan Ahn is a Professor of Department of Life Science, University of Ulsan, South Korea.
