Journal of Bioremediation & Biodegradation
Open Access

Abstract

Traditionally, the contaminated sites are treated by physical and chemical methods, which are often difficult to execute, costly and not ecofriendly. Microbial degradation of chemicals in the environment is an important way to remove the environmentally harmful compounds. Nitro aromatic compounds are raw materials for synthesis of pesticides, pharmaceuticals and plastics. Wide use of these nitro aromatic compounds and their subsequent release leads to environmental pollution. Para-nitro phenol (PNP) is among one such compound found in many different environments. In the present study, we collected the soil at kanchikacharla paddy fields area contaminant with PNP. The soil was serially diluted up to 10-7 and 0.1ml of sample was spread on to 0.5mM PNP minimal media plate and incubated at 370 C. After 3 days of incubation PNP degrading colony was isolated. The organism was identified by16 S rRNA sequencing as Pseudomonas aeuroginosa. The growth kinetics of the organism and PNP degradation were tested at different concentrations of 0.5mM, 0.75mM & 1mM PNP. The growth rate was measured at 600nm shows maximum 0.179 OD value at 60th hour and the degradation of PNP values were 0.162, 0.171 and 0.179mM for 0.5mM, 0.75mM and 1mM respectively. This study facilitated the isolation of organism naturally degrading organism in the micro environment and further studies to isolate genes/ enzymes responsible for degradation of nitro aromatic compounds is under study.

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