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TMany chemicals have been considered as possibly for endocrine disorders described in animals and humans, such as
decreased of sperm count, incidence increased of cancer and congenital malformations in the male reproductive system.
Among the major Endocrine Disruptor Chemicals (EDCs), Bisphenol - A is one substance of greater production and use
around the world.
In vivo
and
in vitro
tests performed with laccase from
Pleurotusostreatus
and
Trametes
sp. show that there
was a reduction of more than 90% of the initial concentration (30mg.L-1), and significant reduction of estrogenic activity,
confirmed by e-screenassay.
In vivo
assays were performed in Erlennmeyer flasks on a rotary shaker at 120 rpm containing:
medium PDB, solution of Bisphenol-A and fungal inoculum, for 54 hours cultivation and removal of aliquots every six hours.
In
in vitro
experiments was added a solution of Bisphenol - A to 72 hours old cultive and maintained for 24 hours, removing
aliquots every six hours. HPLC Chromatographic analysis showed that there was no significant mycelial adsorption. The white
rot fungi
Trametes sp
. and
Pleurotusostreatus
have potential for the removal of Bisphenol - A, reducing significantly its activity
estrogenic.
Biography
Alexandre Nunes Ponezi completed his PhD in Sanitation and Environmental and researcher at Multidisciplinary Center for Chemical, Biological and Agricultural
from State University of Campinas (CPQBA/UNICAMP). He is environmental manager and professor in PhD program from State University of Campinas - Brazil;
and has been serving as a reviewer for international journals.
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