Journal of Biotechnology & Biomaterials
Open Access

Abstract

We surveyed the occurrence of Rice stripe virus (RSV) disease in 860 fields from 27 rice representative areas in July 2014 as nationwide survey for RSV occurrence since 2008. We confirmed occurrence of virus disease in 12 areas, in west coast region including Seosan, Gimje, Buan and Cheorwon. RSV incidence rate of plant in Gimpo and Dangjin was less than 0.01% and 1%, respectively showing similar with the nationwide survey carried out in 2013 whereas incidence rate of field decreased from 12.8% in 2013 to 4.5% in 2014. RSV diagnosis was confirmed by RSV, RDV, RBSDV multiplex diagnostic kits. Mixed infection of these three viruses was not confirmed. Earlier, RSV occurred largely across the southern region. In 2001, incidences of RSV were found in Gangwha and Gyeonggi-do in northern region. In 2007, RSV appeared in west coasts; Buan in Jeollabuk-do region and Seocheon in Choongnam region. After migration of small brown plant hopper from China in 2009, RSV is becoming a pandemic. A nationwide survey was conducted in June and July 2012-2013 in 30 Korean provinces to confirm the RSV sequence variation. A sequence analysis of the nucleocapsid protein (NCP) gene and the intergenic region (IR) of RSV RNA3 was performed. A phylogenetic analysis of the NCP gene and the IR demonstrated that the 33 isolates were closely related to earlier reported isolates from Japan and China, but the secondary IR structure showed geographic preponderance. The results will provide information on the relationships among epidemiology of rice virus disease, climate changes, and insect migration.

Biography

Bong Choon Lee completed PhD from Hokkaido University in Japan and Post-doctoral studies from Chonnam National University in Korea. She is the Senior Researcher of National Institute of Crop Science, RDA, Korea. She has published more than 20 papers in journals and has been serving as an Editorial Board Member of Research in Plant Disease Journal in

Email: Korea. leebc21@korea.kr