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Rapid detection of aerosolized Bacillus spore particles by direct in situ analysis of MALDI-TOF mass spectrometry
7th International Conference and Exhibition on Analytical & Bioanalytical Techniques
Young-Su Jeong, Eugene Chong and Jong Min Lee
Agency for Defense Development, South Korea
Posters & Accepted Abstracts: J Anal Bioanal Tech
Abstract
Accurate and rapid analytical methods are essential for the detection and identification of biological warfare agents (BWA) as well as pathogens. Although various studies have investigated the uses of a matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) for bacterial classification, only a few studies have examined the applicability of method for the identification of BWAs. This study aimed to generate, collect and analyze Bacillus spore aerosol particles of 2-10 �¼m, the optimal size of a BWA. In this process, we developed an apparatus to directly deposit B. globigii aerosols on MALDI target plate wells for rapid MALDI-TOF analysis. Bacillus spore aerosol particles of 2-10 �¼m were rapidly analyzed using direct in situ MALDI-TOF MS without any pretreatment processes. The mass spectra of aerosolized Bacillus spore particles were successfully detected. For real-time detection and identification, a mass spectral database of Bacillus spores was constructed, and an algorithm was developed and applied. Bacillus spore particles were rapidly detected and identified by MS, which can be used for the detection and inspection of BWAs such as B. anthracis spore in the battle field.Biography
Young-Su Jeong has completed his PhD from Korea Advanced Institute for Science and Technology (KAIST) in 2012. He is a Senior Researcher of Agency for Defense Development (ADD), ROK. He has published more than 18 papers in reputed journals.
Email: yiyap@add.re.kr
