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Quantification of phytochemicals and evaluation of antioxidant potential of ethanolic leaf extract of Terminalia bellerica, Terminalia chebula and Emblica officinalis vis-� -vis Triphala
6th World Congress on Biotechnology
Rasna Gupta and Ram Lakhan Singh
Dr. R M L Avadh University, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
Antioxidants are reducing agents which can safely interact with free radicals and terminate the chain reaction initiated by free radicals. There should be a unique balance between generation of ROS and antioxidant defense system in our body. This has led to an increased interest in natural antioxidants from plant sources. The present work was carried out to determine the concentrations of bioactive phytochemicals (ascorbic acid, carotenoid, total phenolic contents, protein and carbohydrate) and evaluation of antioxidant activities of ethanolic leaf extract of Terminalia bellerica (TB), Terminalia chebula (TC), Emblica officinalis (EO) and their formulation Triphala. Among the tested leaf extracts, highest ascorbic acid content was present in EO (118.36 �¼g 100g-1 of fresh weight) followed by Triphala, TB and TC. Highest carotenoid content was present in Triphala (6.53 �¼gg-1 of FW) followed by TB, TC and EO whereas TB leaf extract had highest TPC (215.66 mg-1 of GAE of dry weight) followed by Triphala, TC and EO. Out of three ethanolic leaf extracts, TB showed minimum IC50 for FRSA (58 �¼gml-1), SARSA (38 �¼gml-1), lipid peroxidation (103 �¼gml-1), hydroxyl radical scavenging activity (35 �¼gml-1), FTC activity (117 �¼gml-1) as well as high reducing power (1.77 ASE ml-1). On the basis of our results, it may be concluded that high concentration of phenolic compounds and other bioactive phytochemicals in Triphala and leaf extract of its three constituents are potential source of natural antioxidants.Biography
Rasna Gupta has completed her MSc from C S J M University, Kanpur and she is currently working as a Lecturer in Department of Biotechnology at Dr. R M L Avadh University, Faizabad. She is also pursuing her PhD and has 4 years of research experience. She published 3 research papers and 1 review article in reputed international journals. She has also written a chapter in the book published by CRC press. She has presented her work in three international conferences.
