Journal of Biotechnology & Biomaterials
Open Access

Abstract

Artificial insemination in cattle and buffalo is the common practice and to keep the success at high, the quality of the semen must be good. Seminal plasma is a complex secretion composed by fluids from accessory sex glands, epididymis and testicles and its molecular composition is capable of modulating sperm function. Proteins are the most abundant organic compounds in seminal plasma and play a crucial role in processes related to fertilizing capacity of sperm and can be considered as potential molecular markers of fertility. This study was carried out to assess the protein profile of the Murrah buffalo seminal plasma by using SDS-PAGE and to correlate them with the semen characteristics. Semen samples were collected by a bovine artificial vagina from 8 Murrah buffalo bulls maintained at College of Veterinary Science, N. D. University of Agriculture & Technology, Kumarganj, Faizabad. Semen characteristics were measured using standard procedures. Seminal plasma was separated after centrifugation of semen at 5000 rpm for 10 min. The supernatants were collected and precipitated using nine volumes of cold ethanol and the proteins recovered. These seminal plasma proteins were separated on a 12% SDS-PAGE using standard procedure. A total of 22 protein bands could be identified by the fractionation of seminal plasma protein on the SDS-polyacrylamide gels. Some of these bands were more prominent in some samples. A protein fraction of 25.8 kDa was significantly correlated with progressive motility of sperm. The semen samples that had greater sperm viability showed a prominent fraction of 58 kDa protein in the SDS-PAGE.

Biography

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