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Protein functionalized nanostructured zirconia decorated reduced Graphene oxide based biosensor for food toxin detection
6th World Congress on Biotechnology
Niharika Gupta, Shine Augustine, Suveen Kumar, Saurabh Kumar and Bansi D Malhotra
Delhi Technological University, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
This work describes the fabrication of nanostructured zirconia (~13 nm) decorated reduced graphene oxide (ZrO2-RGO) based immunosensing platform for efficient and label-free detection of food toxin (aflatoxin B1, AFB1). Nanostructured zirconia has been uniformly decorated on reduced graphene oxide via one step, hydrothermal process and further functionalized using 3-amino propyl triethoxy silane (APTES). A thin film of APTES/ZrO2-RGO was obtained through electrophoretic deposition (11 V; 90s) on to an indium tin oxide (ITO) coated glass electrode. Further, anti-AFB1 biomolecules were covalently immobilized on APTES/ZrO2-RGO/ITO electrode through EDC-NHS chemistry. The structural, morphological and electrochemical studies of nanocomposite and the fabricated immunoelectrode were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform-infrared spectroscopy (FT-IR) and cyclic voltammetry techniques. Electrochemical response studies indicate that the immunosensor is capable of detecting AFB1 with a wide linear detection range (0.1-2.5 ng/ml), remarkable sensitivity (1.2 mA mL ng-1 cm-2) and stability of upto 8 weeks.Thus, thisfabricated nanostructured zirconia decorated reduced graphene oxide (ZrO2-RGO) based biosensing platform opens a new window for sensitive detection of food toxin marker.Biography
Email: niharika.gupta990@gmail.com
