Clinical Pharmacology & Biopharmaceutics
Open Access

Abstract

We present here innovative methods to encapsulate proteins into PLGA microparticles/nanoparticles or spherical nanostructured highly porous CaCO3 microparticles using emulsification/extraction process in CO2 medium under mild conditions of pressure and temperature. CaCO3 particles can be obtained by different methods including solution route, evaporation-diffusion route and finally carbonation route via compressed and supercritical CO2. The process employing supercritical CO2 appears to be an efficient method for CaCO3 precipitation and can be used to synthesize hybrid organic-inorganic biomaterials with well-defined properties, according the use of templating biopolymers. In the case of polyester particles, non-toxic solvents, dimethyl isosorbide ether (DMI) and glycofurol (GF) were employed both to precipitate the protein and to dissolve the polymer. Good encapsulation efficiency was obtained with preserved bioactivity of the therapeutic protein. The nano-microparticles were fully characterized in terms physical properties (polymorphism, porosity, chemical composition). In addition, the morphology and surface properties were determined using scanning electron microscopy (SEM) and atomic force microscopy (AFM) respectively. In vitro release study of the protein from nano-microparticles shows the capacity of these systems to control the protein release. Moreover, cytotoxicity study was performed within excellent cytocompatibility of the obtained microparticles. As an example, we described an effective and original process for TGF-�²1 encapsulation into PLGA nano-microparticles. The obtained carriers could be used in many biomedical applications especially for cartilage/bone regeneration combined with hydrogels but could be also used in cancer therapy as tracking systems for cancer cell in brain tumor with other cytokines.

Biography

Email: frank.boury@univ-angers.fr