Journal of Biotechnology & Biomaterials
Open Access

Abstract

Introduction: VVI is a frequent complaint of women of child bearing age. Common causes of VVI include bacterial vaginosis, vulvovaginal candidiasis and trichomoniasis. Toll-like receptors and C-type lectins (CLR) play a major role in recognition of molecular patterns of these pathogens. Mannose Binding Lectin-2 (MBL-2) is asoluble CLR which triggers the complement cascade by binding to carbohydrate moieties on the surface of microorganisms. The MBL-2 codon 54 polymorphism has been associated with increased rate of recurrent vulvovaginal candidiasis. The objective of the present study was to evaluate promoter polymorphism of MBL-2 gene in VVI patients. Materials & Methods: Blood samples were collected from 203 VVI patients as well as matched healthy subjects� in EDTA-coated vials. Three single nucleotide polymorphisms (SNPs) in promoter region i.e., H/L, Y/X and P/Qwere analyzed by Amplification Refractory Mutation System-PCR (ARMS-PCR). The odds ratios (ORs) were calculated using MedCalc software. Results: Significant difference were observed in genotypic as well as allelic frequencies of Y/X polymorphism (p<0.01). X allele was found to be protective factor for VVI patients (OR: 0.5553, 95% CI: 0.4147 to 0.7436, p<0.001). The dominant and codominant models gave the most parsimonious fit for MBL-2Y/X polymorphism (p<0.001). However, no significant differences was observed in genotypic as well as allelic frequencies of H/LandP/Q polymorphisms (p>0.05). Conclusion: The present study showed thatY/Xpolymorphism of MBL-2 gene may be a protective factor for VVI.

Biography

Email: kalianamarta62@gmail.com dr.manpreetdhuna@gmail.com