Journal of Infectious Diseases & Therapy
Open Access

Abstract

Distinct virulence factors of H. pylori have been described: the vacuolatingcytotoxin (vacA), the cytotoxin associated gene
(cagA), the induced by contact with epithelium factor Antigen (iceAgene) and the urease C gene (ureC). This cross sectional
study aimed to investigate the prevalence of the vacA, cagA, iceA and ureC genotypes of H. pylori isolates. 71 biopsy samples
were subjected to PCR, DNA isolation from biopsy was done by using “QIAamp DNA mini kit” from QIAGEN. Isolated
genomic DNAs were processed for PCR. Out of the 71 biopsies screened, 22(31%) samples were positive for H. pylori by PCR.
PCR results showed high proportion of cagA positive (23.94%) followed ure C genes (5.63%) andvac A(1.4%). Prevalence
of cagA was higher in age group 21-40 yrs. (52.94%), followed by 41-60 yrs. (35.29%) and 61-80 yrs. (11.76%).prevalence of
cagAwas higher in females (10/17), compare to males (7/17), statistically significant (p value-0.042 < 0.05). Out of 17 cagA+
stains 9 were causing Gastritis (52.94%), 5 causing reflux esophagitis (29.41%) and 3 were causing duodenal ulcer (17.64%). We
have 0% prevalence of iceAgene, conversely we had 3 peptic ulcer patients and all the three were cagA positive but ice Anegative.
We conclude that Prevalence of cagA genotype is higher in the Anand district compare to other genotypes. We also conclude
that iceA genotype is not a useful marker of virulence in this population and that the progression from gastritis to peptic ulcer
must require some other genes.

Biography

Himani B Pandya has completed her Ph.D. (medical microbiology) from Pramukh Swami Medical College, Karamsad affliated to Sardar Patel University. She
worked as a Lecturer and Head of department (MLT) for 11 years at Shree P.M. Patel college of Paramedical science and technology, Anand, Gujarat. She has
guided 10 M.sc. MLT students for their dissertation work. She is presently working as a Tutor in S.B.K.S medical college, Gujarat in Microbiology Department. She
has published 8 research papers in reputed national and international journal.