Journal of Obesity & Weight Loss Therapy
Open Access

Abstract

Statement of the problem: Many proteins are known as tumour-suppressors, but also as neuro-protectors, endocrine regulators and anti-diabetic substances (1- 3). On the other hand, the role of gangliosides as regulators in many biological functions and as markers in many multi-factor diseases and disorders was proved (4,5). Methodology & theoretical orientation: The titers of specific anti-ganglioside antibodies were assessed in extracts of brain and pancreas from rat. Total lysates from the two anatomic organs were prepared (controls). Equal volumes of them were passed through GSH-agarose columns for separation of molecules with affinity to the reduced form of tri-peptide Glutathione (GSH). Separate aliquots from the organ lysates were mixed with lysates of laboratory-incubated cells, containing additionally-inserted copy of tumour-suppressor gene scgn, coding the hormone-like protein Secretagogin (SCGN), by transfection with appropriate DNA-vectors, containing also GST-tag. The average titers of IgG anti-ganglioside antibodies were determined by ELISA-technique. Findings: The noted tendency about equal and in some cases, of higher average titers of anti-ganglioside antibodies in the samples from both anatomic organs (Fig. 1b, Fig 2b) compared with the average titers of gangliosides in the samples of the same organs (Fig. 1a, Fig. 2a) suggested a possibility about production of immunoglobulins/antibodies by non-lymphoid cells, tissues and organs. probably as one of the steps in the cascade regulatory mechanisms. These results were in agreement with the literature data in this relation (6-8). SCGN and GSH were shown as two target molecules in these interactions. Because the produced antibodies are outside the germinative centres of the specialized lymphoid tissues and organs, control of their function is very important. Conclusion: The possibility for production of immunoglobulins/antibodies by non-lymphoid cells, tissues and organs was proposed, probably as one of the steps in the cascade regulatory mechanisms. In this way, SCGN and GSH could acquire protective functions against degenerative processes, together with preserving of their ant malignant actions, on both cellular and molecular levels.

Biography

Iskra Sainova, PhD, has completed her PhD at the age of 28 years at the Department of Oncovirology to the Institute of Experimental Pathology and Parasitology (IEPP) to Bulgarian Academy of Sciences (BAS) in Sofia, Bulgaria. The main goal has been directed to development of maximally safe methods for application of viral strains for production of gene-engineering anti-malignant and anti-viral vaccines, but also as vectors for transfer of nucleotide sequences. She is assistant professor in the field of molecular biotechnology, molecular and cellular biology.