Toxicology: Open Access
Open Access

Abstract

Resveratrol is known to be an effective chemopreventive agent against multiple tumor cells, but the increasing drug resistance avoids the cancer treatment in oral cavity cancer. In the current study, we investigated the oral anti-tumor activity of resveratrol and its underlying mechanism in Cisplatin-resistant oral cancer CAR cells. Our results demonstrated that resveratrol provoked autophagic cell death to form AVOs and autophagic vacuoles in CAR cells by acridine orange (AO) and monodansylcadaverine (MDC) staining. Inhibitors of PI3K class III (3-MA) and AMP-activated protein kinase (AMPK) (compound c) suppressed the autophagic vesicle formation, LC3-II protein levels and autophagy induced by resveratrol. Z-VAD-FMK (pan-caspase inhibitor) attenuated resveratrol-triggered cleaved caspase-9, cleaved caspase-3 and cell apoptosis. Resveratrol also enhanced phosphorylation of AMPK and regulated autophagy- and pro-apoptosis-related signals in resveratrol-treated CAR cells. Our results indicated that resveratrol is likely to induce autophagic and apoptotic death in drugresistant oral cancer cells and might become a new approach for oral cancer treatment in the near future.

Biography

Chi-Cheng Lu completed his Graduation and Doctorate from National Chung Hsing University (NCHU), Taichung, Taiwan. He worked at the Department of Food Science and Biotechnology of NCHU as a Post-doctoral Fellow under Chair Professor Gow-Chin Yen. He then worked with Research Fellow Dr Jai-Sing Yang of China Medical University Hospital (Taiwan) as a Post-doctoral Research and Project Scientist. His specific interests are focused on targeting tumor cell demise (apoptosis, autophagy and necrosis) and anti-metastatic effect in vitro and in vivo after exposure to phytochemicals and the synthesis of novel compounds. He has also undertaken oxidative stress and functional food research in recent years and is exploring their molecular mechanisms.

Email: a722353@gmail.com