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In Association with
Polymorphism in prolactin promoter region and egg production performance in Kadaknath hens
6th World Congress on Biotechnology
Shiv Kumar Yadav, S K Maurya and Brijendra Mani Yadav
Narendra Deva University of Agriculture & Technology, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
Present study was carried out to study egg production performance and polymorphism of Prolactin gene at 24 bp indel locus at promoter region (PRL24). Egg production performances were recorded as age at first laying (AFE), Body Weight at First Egg (WFE), Mean Egg Weight (MEW) and Total No. of Eggs at 90 days of laying (TEN). DNA was isolated from 2-3 of blood of 20 birds collected from wing vein. PRL24 locus for indel polymorphism was amplified by PCR and the product was resolved on 6% native PAGE for genotyping. The AFE (d), WFE (Kg), MEW (g) and TEN of Kadaknath hens in the present study were found to be 188.00�±0.71, 1.26�±0.03, 42.83�±0.21 and 37.75�±0.59 respectively. The prolactin gene locus PRL24 showed two alleles I & D and three genotypes: II, ID & DD. The frequencies of I and D alleles at this locus were 0.55 & 0.45 respectively. The birds of D allele had a significantly (P<0.05) better TEN than birds of I allele.Biography
Email: dr.shivkumarjnp@gmail.com
