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Besides potential applications in the agriculture field as natural nitrogen fertilizer, N2-fixing cyanobacteria have recently
gained some attentions for new applications linked to the potential production of biologically active molecules or small
molecules such as hydrogen or ammonium for alga fuels. However, few data are available on cultivation of heterocytous
biomass production under photoautotrophic conditions within culture medium deprived of combined nitrogen. The aim of
this research was to investigate kinetic and yield parameters for biomass production by heterocytous cyanobacteria cultures in
laboratory photobioreactors. The Anabaena variabilis strain PCC 7937 was studied using continuous cultures for analyzing the
biomass productivity as a function of the dilution rate. Then we took advantage of chemostat cultures at a fixed dilution rate
for determination of the relation between culture performances at steady-state and some environmental parameters. Optimal
pH of 6.5 was determined for biomass production under N2. The experimental data confirmed that biomass productivity can
be improved by increasing gas transfer efficiency and dissolved inorganic carbon concentration.