Journal of Biotechnology & Biomaterials
Open Access

Abstract

Methicillin resistant Staphylococcus aureus (MRSA) is one of the most important public health problems in many countries. In recent years, the existence of MRSA in foodstuff of animal origin and its transfer among farm animals, foodstuff of animal origin and human beings have been shown with molecular typing studies. The objective of this study was to investigate existence, methicillin resistance (MR) and clonal relationship of Staphylococcus aureus (S. aureus) strains from foodstuff of animal origin consumed in the Samsun region of Turkey. In this study, a total 175 coagulase positive staphylococci (CPS) strains were isolated from meat (n=110), milk (n=56) and fishery products (n=9). From these, 62 S. aureus strains were identified from meat (n=44), milk (n=9) and fishery products (n=9). Identification and MR properties of the isolates were confirmed by PCR technique in which appropriate primers for nuc and mecA gene were used. For detection of MR, we also used minimal inhibitory concentration (MIC) technique. We compared two techniques; although 21 isolates were determined as MRCPS using MIC (â�?¥12 �?¼g), 18 isolates were detected MRCPS using PCR assay. Among these, 15 isolates were identified as MRSA using PCR technique. We investigated only MRSA isolates for the clonal relationship using PFGE method. PFGE typing of the 15 MRSA strains yielded 6 PFGE patterns. Pattern A and E were found to be dominant types in our study. Pattern E consisting of 7 strains was from fishery products. Pattern A consisting of 4 strains was from meat and fishery products. Patterns B, C, D and F were single isolates from milk, meat and milk products, respectively.

Biography

Email: bsiriken@omu.edu.tr, bsiriken@yahoo.com