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In Association with
One-step biosynthesis of �?±-keto acids by the L-amino acid deaminase: Biocatalyst construction and process optimization
8th Euro Biotechnology Congress
Guocheng Du, Long Liu and Jianghua Li
Jiangnan University, China
Posters-Accepted Abstracts: Biotechnol Biomater
Abstract
L-Aminoacidoxidases (LAAOs) which catalyze the stereo specific oxidative deamination of L-aminoacids to �?±-keto acids and ammoniaare flavinadenine dinucleotide containing homodimeric proteins. L-Aminoacidoxidases are widely distributed in diverse organisms and have a range of properties. In thiswork, we systematially engineered the LAAO from Proteus vulgaris as a robust biocatalyst for the efficient one-step biosynthesis of a-keto acids including �?±-keto glutaric acid, �?±-keto- �?³-methylthiobutyric acid and phenylpyruvic acid which are currently produced by multi-step chemical synthesis with heavy environmental pollution. First, the directed evolution of LAAO was done by error-pronepolymerase chain reaction (PCR) and then the site-saturation mutagensis was conducted at the mutation sites of the positive mutants yielded by error-prone PCR. The bio transformaton conditions including biocatalyst concentration, the substrates concentration, temperature and pH and so on. The maximum titers of �?±-keto glutaric acid, �?±-keto-�?³-methylthiobutyric acid and phenylpyruvic acid are12.3 g/L, 63.6 g/ Land 11.8 g/L respectively under the specific optimal conditions. Compared with the traditional multi-step chemical synthesis our one-step biocatalytic production of a-keto acids has an advantage in terms of less environmental pollution and thus has great potential for industrial production of a-keto acids.Biography
Guocheng Du is the Dean of School of Biotechnology, Jiangnan University, China. He is the Distinguished Professor of Changjiang Scholars, Ministry of Education, China. His current main research focus is on bioprocess engineering and metabolic engineering. He has about 300 publications/communications, which include 68 patents, 10 books, 176 original and review papers, etc. He won two Second Prizes of State Science and Technology Awards in 2006 and 2012, respectively. He has been conferred Honorary Doctorate degree from Jiangnan University, China. He is an Associate Editor of Journal of the Science of Food and Agriculture, and Microbial Cell Factories, and Board Member of Bioresource Technology.
Email: gcdu@jiangnan.edu.cn
