Journal of Infectious Diseases & Therapy
Open Access

Abstract

Diarrhea can cause major child mortality in developing countries. E. coli O157:H7 is one of the most important serotypes of enterohemorrhagic Escherichia coli (EHEC) that can cause diarrhea. It is transmitted to humans through food, and creates complications such as uremic hemorrhagic colitis. Currently, the standard method for the detection of E. coli O157:H7 is culture and detection by serology. Recognition by these methods takes more than 36 hours. Thus, access to a test that could detect E. coli O157:H7 in less time is valuable. The aptamers are the oligonucleotides and short single-stranded DNA or RNA or specific proteins that have the ability to specifically bind to target. In this regard, aptamer is used, capable of binding tightly and specifically to target with complex multimeric structures. In this study, a DNA aptamer that can detect E. coli O157:H7 from other similar species was constructed by Cell-SELEX (Systematic Evolution of Ligands by Exponential Enrichment). A library of DNA aptamer was made. Streptavidin coated magnetic beads were used to select specific aptamer. Selected aptamers were amplified by PCR, in each step, then cloned and sequenced. A 117 bp aptamer was selected by six rounds of SELEX method. The aptamer specific binding to E. coli O157:H7 was also calculated by flow cytometry. Using the new aptamer specific molecular probes may be quick and easy to diagnose clinically used E. coli O157:H7 bacterial infection. On the other hand, the present method is simple and cost effective for specific bacterial detection.

Biography

Email: manaoloomi@yahoo.com